Research Institute Research Articles

Needs for Successful Engagement in Telemedicine Among Rural Older US Veterans and Their Caregivers: Qualitative Study.

Telemedicine is an important option for rural older adults who often must travel far distances to clinics or forgo essential care. In 2014, the Geriatric Research, Education, and Clinical Centers (GRECC) of the US Veterans Health Administration (VA) established a national telemedicine network called GRECC Connect. This network increased access to geriatric specialty care for the 1.4 million rural VA-enrolled veterans aged 65 years or older. The use of telemedicine skyrocketed during the COVID-19 pandemic, which disproportionately impacted older adults, exacerbating disparities in specialty care access as overburdened systems shut down in-person services. This surge presented a unique opportunity to study the supports necessary for those who would forgo telemedicine if in-person care were available. In spring 2021, we interviewed veterans and their informal caregivers to (1) elicit their experiences attempting to prepare for a video visit with a GRECC Connect geriatric specialist and (2) explore facilitators and barriers to successful engagement in a telemedicine visit. We conducted a cross-sectional qualitative evaluation with patients and their caregivers who agreed to participate in at least 1 GRECC Connect telemedicine visit in the previous 3 months. A total of 30 participants from 6 geographically diverse GRECC Connect hub sites agreed to participate. Semistructured interviews were conducted through telephone or the VA's videoconference platform for home telemedicine visits (VA Video Connect) per participant preference. We observed challenges and, when needed, provided real-time technical support to facilitate VA Video Connect use for interviews. All interviews were recorded with permission and professionally transcribed. A team of 5 researchers experienced in qualitative research analyzed interview transcripts using rapid qualitative analysis. From 30 participant interviews, we identified the following 4 categories of supports participants described regarding successful engagement in telemedicine, as defined by visit completion, satisfaction, and willingness to engage in telemedicine in the future: (1) caregiver presence to facilitate technology setup and communication; (2) flexibility in visit modality (eg, video from home or a clinic or telephone); (3) technology support (eg, determining device compatibility or providing instruction and on-demand assistance); and (4) assurance of comfort with web-based communication, including orientation to features like closed captioning. Supports were needed at multiple points before the visit, and participants stressed the importance of eliciting the varying needs and preferences of each patient-caregiver dyad. Though many initially agreed to a telemedicine visit because of pandemic-related clinic closures, participants were satisfied with telemedicine and willing to use it for other types of health care visits. To close gaps in telemedicine use among rural older adults, supports must be tailored to individuals, accounting for technology availability and comfort, as well as availability of and need for caregiver involvement. Comprehensive scaffolding of support starts well before the first telemedicine visit.

[Study on the inhibitory and pro-apoptotic effects of different concentrations of total tanshinone alone and in combination with tyrosine kinase inhibitors on human myeloid leukemia cell lines].

Objective: To explore the effect and investigate the molecular mechanism of different concentrations of total tanshinones alone and in combination with tyrosine kinase inhibitors (TKIs) on the proliferation inhibition and apoptosis of human myeloid leukemia cell lines. Methods: K562 and Kasumi-1 cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, and the TKIs-resistant strain K562/T315I cell line was constructed in Molecular Medicine Research Center, Beijing Lu Daopei Institute of Hematology. Logarithmic growth phase cells were taken and divided into intervention groups with total tanshinone of 0, 2.19, 4.38, 8.75, 17.50 and 35.00 μg/ml intervention groups, which were inoculated in 96-well plates at a density of 1×104 cells/well and exposed to the drug for 24 h, and a control group treated with dimethyl sulfoxide was also set up simultaneously. All experiments were repeated independently 3-5 times. The proliferative activity of the cells was assessed using the CCK-8 assay, the apoptotic rates were measured by flow cytometry, and the expression levels of apoptosis-regulating proteins Bcl-2 and Bax were analyzed by Western blotting. The cell lines treated and untreated with total tanshinone were subjected to transcriptome sequencing and gene set enrichment analysis to identify differentially expressed genes. Results: The half-inhibitory concentration (IC50) values of 8.75 μg/ml total tanshinone at 24 h for K562, K562/T315I and Kasumi-1 cells were (4.11±0.02), (4.95±0.04) and (3.98±0.01) μg/ml, respectively. When combined with 0.25 μmol/L imatinib, 8.75 μg/ml total tanshinone could enhance the induction of apoptosis effects on K562 and K562/T315I cell lines. After being treated with 4.38, 8.75, and 17.50 μg/ml of total tanshinone for 24 h, compared with the control group, total tanshinone upregulated the expression level of Bax protein, downregulated the expression level of Bcl-2 protein, and decreased the Bcl-2/Bax ratio (all P<0.05). Total tanshinone inhibited the proliferation-related signaling pathway and DNA damage repair pathway of myeloid leukemia cell lines, and activated the signaling pathway that induces apoptosis in leukemia cells. Conclusion: Different concentrations of total tanshinoneinhibites proliferation and promote apoptosis in K562, Kasumi-1 and TKIs-resistant K562/T315I cell lines, and further enhance the anti-leukemic effect when combined with TKIs.

The roots of errors in adaptive performance: clustering behavioral patterns after the introduction of a change

Purpose The purpose of this study was to investigate work-related adaptive performance from a longitudinal process perspective. This paper clustered specific behavioral patterns following the introduction of a change and related them to retentivity as an individual cognitive ability. In addition, this paper investigated whether the occurrence of adaptation errors varied depending on the type of change content. Design/methodology/approach Data from 35 participants collected in the simulated manufacturing environment of a Research and Application Center Industry 4.0 (RACI) were analyzed. The participants were required to learn and train a manufacturing process in the RACI and through an online training program. At a second measurement point in the RACI, specific manufacturing steps were subject to change and participants had to adapt their task execution. Adaptive performance was evaluated by counting the adaptation errors. Findings The participants showed one of the following behavioral patterns: (1) no adaptation errors, (2) few adaptation errors, (3) repeated adaptation errors regarding the same actions, or (4) many adaptation errors distributed over many different actions. The latter ones had a very low retentivity compared to the other groups. Most of the adaptation errors were made when new actions were added to the manufacturing process. Originality/value Our study adds empirical research on adaptive performance and its underlying processes. It contributes to a detailed understanding of different behaviors in change situations and derives implications for organizational change management.

Prevalence and correlates of anxiety and depressive symptoms after Spontaneous Coronary Artery Dissection (SCAD): a cross-sectional study.

Spontaneous coronary artery dissection (SCAD) is recognised as a particularly stressful cause of heart attack. However few studies have documented the prevalence of post-SCAD anxiety and depressive symptoms, or identified patients most at risk. This study documents the prevalence and correlates of post-SCAD anxiety and depressive symptoms. 310 (95% women) SCAD survivors were recruited by the Victor Chang Cardiac Research Institute from a database of 433 SCAD survivors. Participants completed an online questionnaire to gather demographic, medical and psychosocial information, including the Generalised Anxiety Disorder-7 (GAD-7) and the Patient Health Questionnaire-9 (PHQ-9). Bivariate and multivariate analyses were undertaken to identify the significant demographic, psychosocial and medical correlates of post-SCAD anxiety and depressive symptoms. Time between SCAD and questionnaire completion varied from 2 months to 18 years (mean = 5.5 years; SD = 3.5 years). Rates of anxiety and depressive symptoms were 20.7% (GAD-7 ≥ 10) and 20.9% (PHQ-9 ≥ 10) respectively, and did not vary by time since event. In bivariate analyses, correlates (p < .05) of anxiety and depressive symptoms were absence of a close confidante, financial strain, mental health diagnosis pre-SCAD, comorbid obesity, not being in paid employment (anxiety only), younger age (depression only), and not knowing another SCAD survivor (depression only). Variables retained in multivariate models were absence of a close confidante, financial strain, not being in paid employment, mental health diagnosis pre-SCAD (depression only), and younger age (depression only). This study demonstrated that over one in four SCAD survivors experience either anxiety or depressive symptoms after SCAD, and identified those who may need additional support in their psychological recovery.

A bibliometric analysis of the application of the PI3K-AKT-mTOR signaling pathway in cancer.

PI3K-AKT-mTOR plays as important role in the growth, metabolism, proliferation, and migration of cancer cells, and in apoptosis, autophagy, inflammation, and angiogenesis in cancer. In this study, the aim was to comprehensively review the current research landscape regarding the PI3K-AKT-mTOR pathway in cancer, using bibliometrics to analyze research hotspots, and provide ideas for future research directions. Literature published on the topic between January 2006 and May 2023 was retrieved from the Web of Science core database, and key information and a visualization map were analyzed using CiteSpace and VOSviewer. A total of 5800 articles from 95 countries/regions were collected, including from China and the USA. The number of publications on the topic increased year on year. The major research institution was the University of Texas MD Anderson Cancer Center. Oncotarget and Clinical Cancer Research were the most prevalent journals in the field. Of 26,621 authors, R Kurzrock published the most articles, and J Engelman was cited most frequently. "A549 cell," "first line treatment," "first in human phase I," and "inhibitor" were the keywords of emerging research hotspots. Inhibitors of the PI3K-AKT-mTOR pathway and their use in clinical therapeutic strategies for cancer were the main topics in the field, and future research should also focus on PI3K-AKT-mTOR pathway inhibitors. This study is the first to comprehensively summarize trends and development s in research into the PI3K-AKT-mTOR pathway in cancer. The information that was obtained clarified recent research frontiers and directions, providing references for scholars of cancer management.

Brown manuring for improved crop growth, yield and energetics of wet seeded-rice (Oryza sativa) in the coastal deltaic eco-system

A field experiment was conducted during October 2020–January 2021 at Pandit Jawaharlal Nehru College of Agriculture and Research Institute, Karaikal, Puducherry to study the effect of different seeding rates of [Sesbania bispinosa (Jacq.) W. Wight] (Sesbania) in rice co-culture along with suitable post-emergence herbicides for brown manuring on crop productivity and energetics in wet seeded rice (Oryza sativa L.). The experiment consisted of 10 treatments, viz. T1 ,Sesbania seeding rate for brown manuring (BM) @15 kg/ha with 2,4-D 0.5 kg/ha; T2 , Sesbania seeding rate for BM @20 kg/ha with 2,4-D 0.5 kg/ha; T3 , Sesbania seeding rate for BM @25 kg/ha with 2,4-D 0.5 kg/ha; T4 , Sesbania seeding rate for BM @15 kg/ha with bispyribac sodium 25 g/ha; T5 , Sesbania seeding rate for BM @20 kg/ha with bispyribac sodium 25 g/ha; T6 , Sesbania seeding rate for BM @25 kg/ha with bispyribac sodium 25 g/ha; T7 , rice alone with 2,4-D 0.5 kg/ha; T8 , rice alone with bispyribac sodium 25 g/ha; T9 , hand weeding twice at 20 and 40 DAS (days after sowing); T10, unweeded control in a randomized block design (RBD) with three replications. Sesbania seeding rate for brown manuring (BM) @25 kg/ha + 2,4-D 0.5 kg/ha recorded significantly lower total weed density (73.3 no./m2) and weed dry weight (37.0 g/m2). Significantly higher crop growth rate (CGR) (528.2 g/m2 /day), plant height (132.8 cm) and rice yield (4823 kg/ha) were observed under Sesbania seeding rate for BM @25 kg/ha + 2,4-D 0.5 kg/ha which was comparable with Sesbania seeding rate for BM @25 kg/ha + bispyribac sodium 25 g/ha but superior to treatments without BM and eventually resulted in higher energy productivity (0.59 to 0.60 kg/MJ), energy profitability (7.74 to 7.77 kg/MJ) and the energy use efficiency (8.74 to 8.77). Thus, Sesbania seeding rate @25 kg/ha along with spraying either 2,4-D 0.5 kg/ha or bispyribac sodium 25 g/ha for brown manuring could be recommended in wet seeded rice.

GPR and ERT Exploration in the Western Cemetery in Giza, Egypt

ABSTRACTA geophysical exploration employing ground‐penetrating radar (GPR) and electrical resistivity tomography (ERT) was conducted at the Western Cemetery, Giza, Egypt, in 2021–23 by a joint research team of Higashi Nippon International University, Tohoku University and the National Research Institute of Astronomy and Geophysics (NRIAG), Helwan, Egypt. We believe we found an anomaly: a combination of a shallow structure connected to a deeper structure. The shallow structure, which is L‐shaped in the horizontal plane, 10 m by 10 m, was clearly imaged by GPR. It seems to have been filled with sand, which means it was backfilled after it was constructed. It may have been an entrance to the deeper structure. Its depth is of up to 2 m, measured from the ground surface. Below this structure, ERT reveals a highly electrically resistive anomaly, which extends over an area of about 10 m by 10 m. The depth of the structure is about 5–10 m below the surface. Electrically resistive material in a sand dune can be a mixture of sand and gravel, including sparse spacing or air voids within it. Neither GPR nor ERT could identify the properties of the anomaly.

Halophilic microbial formulations for improving crop productivity and soil health under saline Vertisols of Gujarat

A study was carried out during winter (rabi) seasons of 2018 and 2019 at research farm of ICAR-Central Soil Salinity Research Institute, Regional Research Station, Bharuch, Gujarat to find out the effect of various halophilic microbial formulations in combination of organic manure and chemical fertilizers on winter (rabi) wheat (Triticum aestivum L.) productivity under saline Vertisols. Experiment was conducted in a randomized block design (RBD) comprised of 5 treatments, viz. T1 , Un-inoculated + Vermicompost @2.5 t/ha + 100% RDF (recommended dose of fertilizers); T2 , Un-inoculated + Vermicompost @2.5 t/ha + 75% RDF; T3 , Vermicompost @2.5 t/ha + 75% RDF + Halo-Azo inoculation; T4 , Vermicompost @2.5 t/ha + 75% RDF + Halo-PSB inoculation; and T5 , Vermicompost @2.5 t/ha + 75% RDF + Mix of Halo-Azo and Halo-PSB inoculation, replicated thrice. Results showed that application of different halophilic microbial formulations like Halo-Azo (Azotobacter sp. and Bacillus sp.) and Halo-PSB (consortia of Eneterobacter and Brevibacterium sp.) with vermicompost @2.5 t/ha + 75% RDF (recommended dose of fertilizers), provided at par yield with full dose (120 : 60 : 00; N : P : K kg/ha) of fertilizers, thus able to reduce 25% chemical fertilizer dose. Halo-Azo formulation performed better for improving crop yield as compared to Halo-PSB formulation. Combined application of Halo-Azo and Halo-PSB with vermicompost @2.5 t/ha and 75% RDF performed the best and provided significantly higher grain yield (2.75 t/ha) as compared to other treatments. Single and combined application of Halo-Azo and Halo-PSB with vermicompost @2.5 t/ha and 75% RDF also improved soil health parameters and significant results, i.e. declined EC (2.46 dS/m) and pH (7.33), improved organic carbon (0.69%) and nutrient availability (available N: 299.8 kg/ha and P: 28.7 kg/ha) were obtained. Results showed that integration of halophilic microbial formulations with organics and chemical fertilizers was able to improve wheat yield up to 15% than conventional farming under saline Vertisols of Gujarat.

Effect of phosphorus management on yield and economics under direct seeded rice (Oryza sativa) and blackgram (Vigna mungo) intercropping in a rainfed acidic upland soil

A field experiment was conducted during rainy (kharif) season of 2019 at ICAR-National Rice Research Institute (NRRI), Central Rainfed Upland Rice Research Station (CRURRS), Hazaribagh, Jharkhand to study the effect of phosphorus management on productivity and profitability under direct seeded rice (Oryza sativa L.) (DSR) and blackgram [Vigna mungo (L.) Hepper] intercropping in rainfed acidic upland soil. The experiment was laid-out in a split-plot design comprised 4 methods of cropping, viz. Sole DSR; Sole blackgram; DSR + blackgram (4:1); and DSR + blackgram (4:2) allotted to main-plots and 4 phosphorus management practices, viz. P control (P0 ); Recommended dose of phosphorus (RDP) @17.5 kg P/ha; 50% RDP + phosphate solubilizing bacteria (PSB) @500 g/ha + arbuscular mycorrhiza fungi (AMF) @12.5 kg/ha; and 25% lime requirement (LR) + PSB + AMF in sub-plots. Experiment consisted of 16 treatment combinations, replicated thrice. The rice variety ‘Sahabhagi’ and blackgram variety ‘Uttara’ were used. Among the cropping systems, the highest grain yield was recorded in sole rice (3.84 t/ha) and sole blackgram (1.14 t/ha) which was significantly higher than yields under intercropping in both the ratios. Gross (`96,879/ha) and net returns (`70,603/ha) of sole blackgram were significantly higher than other cropping systems and followed by DSR + blackgram (4:2). Net benefit:cost (B:C) of sole blackgram was maximum (`2.69) and lowest in sole rice (`1.01). Among phosphorus management treatments, 50% RDP + PSB + AMF gave significantly higher net return (`49,440) and net benefit:cost (`1.68). Application of 100% RDP and 25% LR + PSB + AMF gave at par returns and net benefit:cost. It may be suggested that under rainfed uplands of eastern India, farmers may adopt either sole blackgram or intercropping of rice and blackgram in 4:2 over sole DSR for higher productivity and monetary returns. For better phosphorus management in soil they should inoculate PSB and AM fungi along with 50% RDP at the time of sowing.

Research ethics in social science research during health pandemics: what can we learn from COVID-19 experiences?

The COVID-19 pandemic posed many ethical and practical challenges for academic research. Some of these have been documented, particularly in relation to health research, but less attention has been paid to the dilemmas encountered by educational and social science research. Given that pandemics are predicted to be more frequent, it is vital to understand how to continue crucial research in schools and other learning communities. This article therefore focuses specifically on research ethics in educational and social science during the pandemic of 2020–2022. The research involved interviews and workshops with University College London (UCL) academics, professional staff and graduate students and encompassed those involved in reviewing ethics applications, researchers dealing with ethics in projects that continued despite disruptions caused by COVID-19, and successful research projects specifically designed to study the effects of COVID-19 in various contexts. The article discusses some of the crucial knowledge and practical experiences that were accumulated. The operational and epistemological lessons learned from this particular institution may have wider relevance to research ethics processes in higher education environments where academics and students are grappling with post-COVID-19 ethical dilemmas and inform broader debates about how research institutions can build institutional knowledge to improve practices of ethics review at the times of health emergencies in future. Our evidence points to the significance of inter- and multidisciplinary, collaborative approaches that flatten institutional hierarchies and to the crucial role played by professional staff. In addition, we argue that ethics review processes must be underpinned by critical debates about wider issues of unequal power relationships between research partners, the nature of knowledge production, ownership and utilisation. To enhance equity and epistemic justice in research practices, ethics education should be an ongoing integral part of research ethics within research institutions.

Effects of Packaging Materials on Food Quality and Shelf Life in Australia

Purpose: The aim of the study was to analyze the effects of packaging materials on food quality and shelf life in Australia. Methodology: This study adopted a desk methodology. A desk study research design is commonly known as secondary data collection. This is basically collecting data from existing resources preferably because of its low cost advantage as compared to a field research. Our current study looked into already published studies and reports as the data was easily accessed through online journals and libraries. Findings: In Australia, advanced packaging technologies such as modified atmosphere packaging (MAP) and active packaging extend the shelf life of perishables by inhibiting microbial growth. Sustainable packaging options are gaining traction, balancing environmental concerns with maintaining food safety. Intelligent packaging with condition indicators enhances consumer decisions by providing food safety information. Strict Australian regulations ensure that packaging materials meet safety standards, supporting food quality preservation. Unique Contribution to Theory, Practice and Policy: Diffusion theory, mass transfer theory &amp; shelf life prediction models may be used to anchor future studies on analyze the effects of packaging materials on food quality and shelf life in Australia. Practical recommendations should emphasize the adoption of innovative packaging technologies tailored to specific food products and storage conditions. Policymakers should prioritize initiatives aimed at fostering collaboration between industry stakeholders, research institutions, and regulatory bodies to address pressing challenges in food packaging.

211 Analysis of microbial communities from a commercial-size swine manure pit and their association with production of volatile organic compounds

Abstract Global pollution and emission concerns have been linked to concentrated animal feeding operations (CAFOs). Indeed, the U.S Environmental Protection Agency (US EPA) warns that there is a significant danger to human health, soil, and water quality from CAFOs. Volatile organic compounds (VOCs), which are amongst air contaminants that pose a serious risk, are abundantly produced in swine manure pits. While VOC emissions from swine manure have been well studied, the microbial species responsible for their production remain mostly uncharacterized. Thus, a deeper comprehension of the microorganisms responsible for VOC production in swine manure pits may help in the development of management approaches that target the microbiome in these environments. In this context, this study aimed to identify the major bacterial species populating the manure pit of a commercial-size swine wean-to-finish research facility. Manure collected from two sampling locations at different time points were analyzed using the 16S rRNA gene through PCR-amplification of the V1-V3 regions from microbial genomic DNA, followed by Illumina MiSeq 2X300 sequencing. An analysis of the most highly represented microorganisms identified 24 bacterial Operational Taxonomic Units (OTUs), with only four showing a species-level nucleotide sequence identity to currently known or valid species. As these results indicated that the majority of bacterial species from this manure pit were unknown, a metagenomics approach was used to assemble partial to full length genomes from the two most abundant OTUs: Pit_9-00239 [5.09% - 19.44%; Clostridium saccharoperbutylacetonicum (96.60%)] and Pit_ 7-00035 [5.58% - 17.30%; Bacteroides eggerthii (87.66%)]. From genome assembly analysis, 979 contigs were generated for a combined length of 12,367,510 bp, which together included 21,776 coding sequences. Predicted metabolic functions from these contig sets included pathways involved in carbohydrate utilization (glycolysis), vitamin synthesis (riboflavin), and butyrate production (volatile fatty acids). Notably, an enzyme responsible for the production of indole, a common VOC produced from swine manure pits, was also identified. Together, the results from this analysis provide insights on metabolic capabilities of abundant bacterial species from manure pits. These can in turn be used for the future development of management strategies or products that can reduce VOC emissions that result from swine CAFO manure storage.

Project Management Competencies in AI-Driven Environments: A Qualitative Assessment

The objective of this paper is to provide an evaluation of project management skills and competence in AI-driven environments as an essential scope due to their pivotal role in producing effective outcomes in the fast-paced world of rapidly evolving technology. This study employs a cross-sectional research design and qualitative survey methodology to examine project management in the context of AI integration. The study involved a broad base, which includes players from various sectors like technology, finance, health, and manufacturing, so its findings and recommendations are all-encompassing. This study achieves this by utilizing datasets obtained from industry-leading AI companies, academic research institutions, and governmental agencies. These datasets comprise project management metrics, AI implementation case studies, and surveys conducted among project managers and stakeholders in AI-driven industries in addition to literature sources, using sophisticated statistical techniques. The findings show and present the changes that the practice of project management faces in the era of AI and provide the most helpful guidance for project managers, stakeholders, and organisations that strive to cope with this dynamic and changing environment. The research result shows that by emphasising skill-based development programmes, cultivating an innovation- friendly culture, and adopting AI-driven technologies, organisations can be at the forefront of technological growth, gaining a competitive advantage in the highly dynamic business environment. The research shows that in the future, the enhanced use of AI technologies will keep changing the project management landscape. This research strengthens the theoretical underpinnings of project management in AI-powered projects and ensures the enhancement of project management's actual efficacy in response to technological advancements. This will aid project managers deliver the most important skills necessary for effective project management in AI-driven environments.

Changes in the epidemiology and clinical characteristics of viral gastroenteritis among hospitalized children in the Mainland of China: a retrospective study from 2016 to 2020

BackgroundAcute gastroenteritis (AGE) causes significant morbidity in children worldwide; however, the disease burden of children hospitalized with viral gastroenteritis in China has been rarely described. Through this study, we analyzed the data of hospitalized children with viral gastroenteritis to explore the changes in the epidemiology and clinical characteristics of viral gastroenteritis in the mainland of China.MethodsData were extracted from Futang Children's Medical Development Research Center (FRCPD), between 2016 and 2020, across 27 hospitals in 7 regions. The demographics, geographic distribution, pathogenic examination results, complications, hospital admission date, length of hospital stays, hospitalization charges and outcomes were collected and analyzed.ResultsViral etiological agents included rotavirus (RV), adenovirus (ADV), norovirus (NV) and coxsackievirus (CV) that were detected in 25,274 (89.6%), 1,047 (3.7%), 441 (1.5%) and 83 (0.3%) cases. There was a higher prevalence of RV and NV infection among children younger than 3 years of age. RV and NV had the highest detection rates in winter, while ADV in summer. Children with viral gastroenteritis were often accompanied by other diseases, such as myocardial diseases (10.98–31.04%), upper respiratory tract diseases (1.20–20.15%), and seizures (2.41–14.51%). Among those cases, the co-infection rate with other pathogens was 6.28%, with Mycoplasma pneumoniae (M. pneumoniae), Epstein-Barr virus (EBV), and influenza virus (FLU) being the most common pathogens. The median length of stay was 5 days, and the median cost of hospitalization corresponded to587 US dollars.ConclusionsThis finding suggests that viral gastroenteritis, especially those caused by RV, is a prevalent illness among younger children. Co-infections and the presence of other diseases are common. The seasonality and regional variation of viral etiological agents highlight the need for targeted prevention and control measures. Although viral gastroenteritis rarely leads to death, it also results in a significant economic burden on healthcare systems.

PSII-21 Feed intake and growth of young turkeys in the brood when supplemented with Viribus Essentials, a blend of botanical extracts and natural flavors

Abstract The objective of this experiment was to determine the effect of adding Viribus Essentials (VB) on top of standard commercial brood diets on the performance and survivability of young turkeys in a large-scale commercial research facility. A total of 1,360 hybrid turkey toms were randomly assigned to 2 dietary treatments in a completely randomized design for a 42-d trial. There were 85 turkeys per pen and 8 replicate pens per treatment. The 2 dietary treatments were 1) CONTROL a standard set of commercial turkey brood rations (Starter, Grower), and 2) CONTROL + 1,362 grams per ton of VB in Starter and Grower. Body weight and feed disappearance were recorded by pen for d 0 to 42 to calculate average daily gain, average daily feed intake, and feed conversion ratio. The Grubb’s test was used to check data for outliers with P &amp;lt; 0.05 as the level of significance for eliminating a replicate; no replicates were removed. Data were used to calculate all parameters and were analyzed using the GLM procedure in Minitab with Fisher’s t-test determining differences between treatments. Results from this trial are shown in Table 1. VB improved feed intake by 4.6% (P &amp;lt; 0.05), which resulted in improved average daily gain (ADG) by 3.8% (P &amp;lt; 0.01), with no change in feed conversion ratio (FCR) or Mortality adjusted FCR. Survivability was numerically increased by 1.1% (P = 0.367). Overall, the supplementation of VB increases the body weight of the turkey toms at the end of the brood by 4.7% (P &amp;lt; 0.01) which is an important indicator for the lifetime performance of turkeys.

School of Nursing Climate Commitment: Nursing Faculty Bring Climate to the Classroom

In 2021, the American Association of Colleges of Nursing (AACN) added “the impact of climate change on environmental and population health” into The Essentials: Core Competencies for Professional Nursing Education. Presently, little guidance exists for nursing faculty new to climate education. The year prior, the Nurses Climate Challenge (NCC)—a campaign to educate 50,000 health professionals about health impacts of climate change—launched the School of Nursing Commitment through a series of focus groups and collaborative content development. With an aim of increasing access to knowledge and tools to support education about the health impacts of climate change, the NCC Commitment partners with nursing schools and provides a community of practice. Partner schools use NCC resources in courses and report the number of students educated. Within three years, 61 nursing schools in 30 states joined the Commitment. Participants included academic health centers, research institutions, multi-state schools, and small private colleges, and programs ranged from AD to PhD. Faculty (1) integrated resources into didactic and clinical settings, such as population or organ-system content, leadership, and policy; and (2) used resources to support assignments. In four years, faculty reported educating over 37,700 students, using NCC resources in 439 educational sessions. The Commitment may be valuable for faculty fulfilling AACN Essentials by bringing climate change to the classroom, community, and bedside. Furthermore, the Commitment may be a replicable model for health professional education and inspiring action on climate change.

Risk factors and prognostic significance of early postoperative complications for patients who underwent pneumonectomy for lung cancer

BackgroundAlthough pneumonectomy has relatively high mortality and morbidity rates, it remains valid in the surgical treatment of lung cancer. This study aims to evaluate the prognostic significance of postoperative complications after pneumonectomy and demonstrate the risk factors related to early postoperative complications.MethodsPatients who underwent pneumonectomy for non-small cell lung cancer between January 2008 and May 2021 were included in the study. Factors related to the development of early postoperative complications and overall survival were evaluated by univariate and multivariate analyses.ResultsA total of 136 patients were included in the study. Early postoperative complications were seen in 33 (24.3%) patients and late postoperative complications in 7 (5.1%) patients. The amount of cigarette smoking, and the operation side were the independent variables that affect the development of early postoperative complications. In multivariate analysis, smoking amount and pericardial invasion were associated with the development of postoperative hemorrhage, and advanced age was associated with the development of postoperative pneumonia.ConclusionsEarly postoperative complications have a negative effect on the prognosis after pneumonectomy therefore careful patient selection and preoperative risk assessment are essential to minimize the occurrence of complications and improve patient outcomes.Trial registrationThis observational study was approved by the (Ethical Committee of Dr. Suat Seren Chest Diseases and Chest Surgery Education and Research Center) Institutional Review Board of our center (E-49109414-604.02.02-218625439).

Rapid detection of the source of a Listeria monocytogenes outbreak in Switzerland through routine interviewing of patients and whole-genome sequencing.

Listeriosis is a notifiable disease in Switzerland. In summer 2022, the Swiss Federal Office of Public Health noticed an increase in reports of listeriosis cases, indicating a possible ongoing outbreak. Here we present the approaches applied for rapidly confirming the outbreak, detecting the underlying source of infection and the measures put in place to eliminate it and contain the outbreak. For close surveillance and early detection of outbreak situations with their possible sources, listeriosis patients in Switzerland are systematically interviewed about risk behaviours and foods consumed prior to the infection.Listeria monocytogenesisolates derived from patients in medical laboratories are sent to the National Reference Laboratory for Enteropathogenic Bacteria and Listeria, where they routinely undergo whole-genome sequencing. Interview and whole-genome sequencing data are continuously linked for comparison and analysis. In summer 2022, 20 patient-derivedL. monocytogenesserotype 4b sequence type 388 strains were found to belong to an outbreak cluster (≤10 different alleles between neighbouring isolates) based on core genome multilocus sequence typing analysis. Geographically, 18 of 20 outbreak cases occurred in northeastern Switzerland. The median age of patients was 77.4 years (range: 58.1-89.7), with both sexes equally affected. Rolling analysis of the interview data revealed smoked trout from a local producer as a suspected infection source, triggering an on-site investigation of the production facility and sampling of the suspected products by the responsible cantonal food inspection team on 15 July 2022. Seven of ten samples tested positive forL. monocytogenesand the respective cantonal authority ordered a ban on production and distribution as well as a product recall. The Federal Food Safety and Veterinary Office released a nationwide public alert covering the smoked fish products concerned. Whole-genome sequencing analysis confirmed the interrelatedness of theL. monocytogenessmoked trout product isolates and the patient-derived isolates. Following the ban on production and distribution and the product recall, reporting of new outbreak-related cases rapidly dropped to zero. This listeriosis outbreak could be contained within a relatively short time thanks to identification of the source of contamination through the established combined approach of timely interviewing of every listeriosis patient or a representative and continuous molecular analysis of the patient- and food-derivedL. monocytogenesisolates. These findings highlight the effectiveness of this well-established, joint approach involving the federal and cantonal authorities and the research institutions mandated to contain listeriosis outbreaks in Switzerland.

Rice breeding for higher crop productivity (a review)

The main global trends in the breeding of rice cultivars and hybrids are higher plant productivity, adaptability to cultivation conditions, quality of products, and resistance to environmental stressors, including diseases.Efforts of leading breeding centers in the Philippines and China to further increase the rice yield are associated with a change in the plant morphotype: shorter plants with erectoid leaves and large panicles with a high number of grains.The International Rice Research Institute (IRRI) has developed rice cultivars that under favorable conditions in the tropical zone produce a grain yield of up to 10–11 t/ha, with Kecon = 0.5. A heterotic hybrid of rice was obtained in China with a yield of 13.9 t/ha; it has a new plant morphotype (New Plant Type) and a growing season of 163 days. The hybrid is classified as “super rice”.Modern breeding programs are aimed at producing cultivars of “Green Super Rice” using pedigree methods and backcrossing for introgression of target genes through DNA markers, QTL mapping, pyramiding, and recurrent selection.Russian breeders have traditionally produced rice cultivars adapted to local conditions, with a growing season of no more than 125 days. In the 1980s, a program was launched to develop a high-yielding rice cultivar with erectoid leaves for condensed planting. As a result of multiple stepwise hybridization and targeted selection, new source material with target traits of “super rice” was released. Cv. ‘Polyus-5’ with a new plant morphotype was obtained on its basis. This cultivar was included in the State Register and from 2023 approved for commercial production. During the field trials, ‘Polyus-5’ yielded a maximum of 1380 g/m2 over 120 days of the growing season, under the conditions of condensed sowing and increased mineral nutrition.

Severe morbidity and hospital-based mortality from Rift Valley fever disease between November 2017 and March 2020 among humans in Uganda

BackgroundRift Valley fever (RVF) is a zoonotic viral disease of increasing intensity among humans in Africa and the Arabian Peninsula. In Uganda, cases reported prior to 2016 were mild or not fully documented. We report in this paper on the severe morbidity and hospital-based mortality of human cases in Uganda.MethodsBetween November 2017 and March 2020 human cases reported to the Uganda Virus Research Institute (UVRI) were confirmed by polymerase chain reaction (PCR). Ethical and regulatory approvals were obtained to enrol survivors into a one-year follow-up study. Data were collected on socio-demographics, medical history, laboratory tests, potential risk factors, and analysed using Stata software.ResultsOverall, 40 cases were confirmed with acute RVF during this period. Cases were not geographically clustered and nearly all were male (39/40; 98%), median age 32 (range 11–63). The median definitive diagnosis time was 7 days and a delay of three days between presumptive and definitive diagnosis. Most patients (31/40; 78%) presented with fever and bleeding at case detection. Twenty-eight (70%) cases were hospitalised, out of whom 18 (64%) died. Mortality was highest among admissions in regional referral (11/16; 69%) and district (4/5; 80%) hospitals, hospitalized patients with bleeding at case detection (17/27; 63%), and patients older than 44 years (9/9; 100%). Survivors mostly manifested a mild gastro-intestinal syndrome with nausea (83%), anorexia (75%), vomiting (75%), abdominal pain (50%), and diarrhoea (42%), and prolonged symptoms of severe disease including jaundice (67%), visual difficulties (67%), epistaxis (50%), haemoptysis (42%), and dysentery (25%). Symptom duration varied between two to 120 days.ConclusionRVF is associated with high hospital-based mortality, severe and prolonged morbidity among humans that present to the health care system and are confirmed by PCR. One-health composite interventions should be developed to improve environmental and livestock surveillance, prevent infections, promptly detect outbreaks, and improve patient outcomes.